circRNA basic information
circBase ID: hsa_circ_0004771
Name: hsa_circ_NRIP1
Synonym: circ_NRIP1
Host Gene: NRIP1
Genomic location(hg19): chr21:16386664-16415895:-
Genomic location(hg38): chr21:15014343-15043574:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0005580
MONDO name: esophageal squamous cell carcinoma
Disease details: esophageal squamous cell carcinoma
Disease DO ID:
3748
Disease MeSH ID:
C562729; D000077277
Disease NCIt ID:
C4024
Disease ICD11 ID:
1417891145
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissues; adjacent normal tissues

Cell lines:

KYSE30; KYSE450; HET-1A

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: SEMA4D, PI3K, AKT
Associated microRNA: miR-595
Biological function: promotes cell growth/proliferation and suppresses apoptosis; promotes migration, invasion and EMT-like changes; promotes tumor growth in vivo
Molecular mechanism: ceRNA mechanism: circ_NRIP1 sponges miR-595 to de-repress SEMA4D, leading to activation of PI3K/AKT signaling
Biological pathway or process:

PI3K/AKT (promotes); apoptosis (inhibits); proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); ceRNA regulation (promotes)

Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Transfection; Colony Formation Assay; MTT; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; Luciferase Reporter Assay; In Vivo Animal Model; Clinical Sample Validation; Bioinformatics Analysis

Clinical significance:

circ_NRIP1 expression was associated with TNM stage

Description:

circ_NRIP1 (hsa_circ_0004771) is up-regulated in ESCC tissues and cell lines and acts as an oncogenic circRNA. It sponges miR-595 to increase SEMA4D, thereby promoting proliferation, migration/invasion and EMT-like changes while inhibiting apoptosis, and it supports tumor growth in a cell line-derived xenograft model via PI3K/AKT pathway activation.

Confidence score:

0.7181

Other information
Title:

circ_NRIP1 is oncogenic in malignant development of esophageal squamous cell carcinoma (ESCC) via miR-595/SEMA4D axis and PI3K/AKT pathway.

Journal: Cancer cell international
Published: 2021
PubMed ID: 33957921
Study type:

combined biological and clinical study

Data availability: available from the corresponding author on reasonable request
Code availability: -