| Expression pattern: |
DN |
| Associated gene: |
JUNB |
| Associated microRNA: |
miR-30a-5p |
| Biological function: |
attenuates trophoblast cell dysfunction and inflammation; promotes proliferation, migration and invasion; inhibits apoptosis; reduces secretion of pro-inflammatory cytokines (TNF-alpha, IL-6, IL-8) |
| Molecular mechanism: |
ceRNA sponge mechanism: circPUM1 sponges miR-30a-5p to positively regulate JUNB |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); inflammation (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; Transwell Assay; Annexin V/PI Flow Cytometry; Western Blot; ELISA |
| Clinical significance: |
potential target/biomarker for RSA diagnosis and treatment |
| Description: |
circPUM1 is down-regulated in RSA placental tissues. Functionally, circPUM1 supports trophoblast proliferation/migration/invasion and suppresses apoptosis and inflammatory cytokine secretion by acting as a ceRNA sponge for miR-30a-5p to upregulate JUNB. |
| Confidence score: |
0.7045 |