circRNA basic information
circBase ID: hsa_circ_0087862
Name: -
Synonym: -
Host Gene: -
Genomic location(hg19): chr9:110068659-110081160:+
Genomic location(hg38): chr9:107306378-107318879:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer
Disease DO ID:
3908
Disease MeSH ID:
D002289
Disease NCIt ID:
C2926
Disease ICD11 ID:
-
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

NSCLC tissues; adjacent normal tissues; xenograft tumor tissues

Cell lines:

NCI-H1359; A549; BEAS-2B; H1650; H1975; HCC827

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: RAB3D, AGO2
Associated microRNA: miR-1253, miR-766-5p, miR-223-3p, miR-3139
Biological function: Promotes NSCLC cell viability, migration and invasion; inhibits apoptosis; promotes tumor growth in vivo.
Molecular mechanism: Acts as a ceRNA (miRNA sponge) for miR-1253, thereby relieving repression of RAB3D and promoting NSCLC progression.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); ceRNA regulation (promotes)

Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Annexin V/PI Flow Cytometry; Wound Healing Assay; Transwell Assay; Western Blot; IHC (Immunohistochemistry); TUNEL; In Vivo Animal Model; Clinical Sample Validation; Cohort Study; Survival Analysis; Bioinformatics Analysis

Clinical significance:

High hsa_circ_0087862 expression was associated with poor prognosis and low 5-year survival in NSCLC patients.

Description:

hsa_circ_0087862 is up-regulated in NSCLC and functions as an oncogenic circRNA. It promotes proliferation/viability, migration and invasion while inhibiting apoptosis, and it enhances xenograft tumor growth. Mechanistically, it sponges miR-1253 to increase the downstream target RAB3D, and higher circRNA expression is associated with poorer patient survival.

Confidence score:

0.8282

Other information
Title:

CircRNA hsa_circ_0087862 Acts as an Oncogene in Non-Small Cell Lung Cancer by Targeting miR-1253/RAB3D Axis.

Journal: OncoTargets and therapy
Published: 2020
PubMed ID: 32308420
Study type:

combined biological and clinical study

Data availability: -
Code availability: -