| Expression pattern: |
UP |
| Associated gene: |
EIF4A3, c-Myc mRNA, c-Myc |
| Associated microRNA: |
- |
| Biological function: |
promotes proliferation and migration; promotes tumor growth; promotes cell cycle progression |
| Molecular mechanism: |
binds EIF4A3 and recruits it to enhance c-Myc mRNA stability, thereby increasing c-Myc protein levels (post-transcriptional regulation) |
| Biological pathway or process: |
mRNA stability (promotes); proliferation (promotes); migration (promotes); cell cycle (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RNA Pull-Down; RIP (RNA Immunoprecipitation); IF (Immunofluorescence); Transfection; MTT; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Cell Cycle Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry) |
| Clinical significance: |
High expression of hsa_circ_0068631 was positively associated with TNM stage, tumor size, lymph node metastasis and distant metastasis |
| Description: |
hsa_circ_0068631 is upregulated in breast cancer tissues and cell lines and promotes proliferation, migration, and tumor growth. Mechanistically, it binds and recruits the RBP EIF4A3 to c-Myc mRNA, increasing c-Myc mRNA stability and elevating c-Myc protein expression, thereby driving breast cancer progression. |
| Confidence score: |
0.8588 |