circRNA basic information
circBase ID: hsa_circ_0000028
Name: hsa_circ_USP48
Synonym: circUSP48
Host Gene: USP48
Genomic location(hg19): chr1:22041881-22048257:-
Genomic location(hg38): chr1:21715388-21721764:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0009807
MONDO name: osteosarcoma
Disease details: osteosarcoma
Disease DO ID:
 3347
Disease MeSH ID:
 D012516
Disease NCIt ID:
 C9145
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

OS tissues; healthy control tissues; tumor tissues
Cell lines:

hFOB1.19; MG63; HOS; 143B
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: CYR61, AGO2
Associated microRNA: miR-365
Biological function: Promotes OS cell proliferation, colony formation, motility, invasion, malignant behavior, and tumor growth.
Molecular mechanism: circUSP48 acts as a miR-365 sponge to upregulate CYR61 expression, forming a circUSP48/miR-365/CYR61 regulatory axis in OS.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); ceRNA regulation (other)
Detected method:
Q
M
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; Microarray; FISH / smFISH; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; Wound Healing Assay; IHC (Immunohistochemistry); IF (Immunofluorescence); Western Blot; In Vivo Animal Model; Bioinformatics Analysis
Clinical significance:

circUSP48 expression was significantly correlated with tumor size and TNM stage, and circUSP48 was proposed as a potential therapeutic target for OS.
Description:

circUSP48/hsa_circ_0000028 is up-regulated in osteosarcoma tissues and cells and promotes malignant phenotypes including proliferation, migration, invasion, colony formation, and tumor growth. Mechanistically, circUSP48 sponges miR-365 to increase CYR61 expression, forming a circUSP48/miR-365/CYR61 axis that contributes to OS progression. Its expression is associated with tumor size and TNM stage, suggesting potential therapeutic relevance.
Confidence score:

0.8748
Other information
Title:

CircUSP48 promotes malignant behavior by regulating CYR61 via miR-365 in osteosarcoma.

Journal: Functional & integrative genomics
Published: 2023
PubMed ID: 37553503
Study type:

combined biological and clinical study
Data availability: GSE96964; The data are available from the corresponding author upon request; https:// doi. org/ 10. 1007/ s10142-023-01197-8
Code availability: -