| Expression pattern: |
DN |
| Associated gene: |
ATG5-ATG12, ATG7, LC3-II |
| Associated microRNA: |
- |
| Biological function: |
Inhibits PCa proliferation by blocking autophagy; knockdown promotes proliferation and tumor growth. |
| Molecular mechanism: |
Knockdown enhances autophagy (increased ATG5-ATG12, ATG7, LC3-II; increased autophagy flux), and autophagy inhibition (CQ) rescues the proliferation promoted by hsa_circ_0001747 knockdown. |
| Biological pathway or process: |
autophagy (inhibits); proliferation (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Sanger Sequencing; Back-Splice Junction PCR / divergent primers PCR; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; Colony Formation Assay; Western Blot; IF (Immunofluorescence); In Vivo Animal Model |
| Clinical significance: |
High expression of hsa_circ_0001747 predicts longer BCR-free time and is a favorable prognostic factor for BCR in PCa. |
| Description: |
hsa_circ_0001747 (from MKLN1) is down-regulated in prostate cancer and acts as a protective factor: higher expression predicts longer BCR-free survival. Functionally, its knockdown enhances autophagy flux and promotes PCa cell proliferation and xenograft growth, while autophagy inhibition (CQ) mitigates this effect. |
| Confidence score: |
0.6634 |