| Expression pattern: |
UP |
| Associated gene: |
SDC2, syntenin-1, NF-kappaB, MMP9 |
| Associated microRNA: |
miR-9-5p |
| Biological function: |
Promotes cervical cancer cell proliferation, migration, invasion and tumor growth; acts as an oncogenic circRNA and potential therapeutic target. |
| Molecular mechanism: |
circNFATC3 functions as a ceRNA/miRNA sponge for miR-9-5p, thereby upregulating SDC2 and downstream syntenin-1, NF-kappaB and MMP9 signaling to promote cervical cancer progression. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); NF-kappaB (promotes); metastasis (promotes); other pathway/process (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; Microarray; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot; Bioinformatics Analysis |
| Clinical significance: |
circNFATC3 expression was positively correlated with tumor size and stromal invasion; it may serve as an indicator for treatment choice and as a therapeutic target for locally advanced cervical cancer. |
| Description: |
circNFATC3 (hsa_circ_0005615), derived from NFATC3, is upregulated in cervical cancer tissues and associated with larger tumor size and stromal invasion. It promotes cervical cancer cell proliferation, migration, invasion and xenograft tumor growth by sponging miR-9-5p, thereby upregulating SDC2 and downstream syntenin-1/NF-kappaB/MMP9 signaling. The study proposes circNFATC3 as an oncogenic circRNA and a potential therapeutic target, especially for advanced cervical cancer or LACC. |
| Confidence score: |
0.866 |