| Expression pattern: |
UP |
| Associated gene: |
STMN1 |
| Associated microRNA: |
miR-223 |
| Biological function: |
promotes GBC cell proliferation, cell cycle progression, tumor growth, and Warburg effect |
| Molecular mechanism: |
ceRNA sponging of miR-223 to upregulate STMN1 |
| Biological pathway or process: |
proliferation (promotes); cell cycle (promotes); glycolysis (promotes); oxidative phosphorylation (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Nuclear-Cytoplasmic Fractionation; RNase R Treatment; FISH / smFISH; Sanger Sequencing; Transfection; CCK8; Colony Formation Assay; Cell Cycle Assay; Luciferase Reporter Assay; RNA Pull-Down; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
circbeta-catenin expression was associated with advanced TNM stage and poor prognosis; higher expression predicted shorter overall survival and was an independent prognostic factor |
| Description: |
circbeta-catenin is up-regulated in gallbladder cancer and mainly localizes to the cytoplasm. It promotes proliferation, cell-cycle progression, tumor growth, and the Warburg effect by sponging miR-223 to increase STMN1 expression, and high circbeta-catenin is associated with worse overall survival. |
| Confidence score: |
0.8508 |