| Expression pattern: |
DN |
| Associated gene: |
TJP1, AGO2 |
| Associated microRNA: |
miR-145-5p |
| Biological function: |
Suppresses cell proliferation, migration, and invasion in Wilms’ tumor. |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-145-5p, thereby upregulating TJP1 via the circCDYL/miR-145-5p/TJP1 axis. |
| Biological pathway or process: |
proliferation (inhibits); migration (inhibits); invasion (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Cell Cycle Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Bioinformatics Analysis; Clinical Sample Validation |
| Clinical significance: |
Increased circCDYL expression in WT had correlation with smaller tumor size and lower clinicopathologic stage; suggested as a novel biomarker and therapeutic target for WT treatment. |
| Description: |
circCDYL is down-regulated in Wilms’ tumor and functions as a tumor suppressor. It sponges miR-145-5p to relieve repression of TJP1, thereby inhibiting WT cell proliferation, migration, and invasion, with validation in vitro and in a cell line-derived xenograft model. |
| Confidence score: |
0.843 |