| Expression pattern: |
UP |
| Associated gene: |
PGK1, AGO2 |
| Associated microRNA: |
miR-296-3p |
| Biological function: |
Promotes CRC cell proliferation, invasion, glycolysis and tumor growth, and inhibits cell apoptosis. |
| Molecular mechanism: |
circ_0087862 acts as a ceRNA by sponging miR-296-3p to regulate PGK1 expression. |
| Biological pathway or process: |
proliferation (promotes); invasion (promotes); glycolysis (promotes); apoptosis (inhibits); ceRNA regulation (other); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; In Vivo Animal Model; IHC (Immunohistochemistry); Bioinformatics Analysis |
| Clinical significance: |
Circ_0087862 might act as a potential target for CRC therapy. |
| Description: |
circ_0087862 is up-regulated in colorectal cancer tissues and cells and functions as an oncogenic circRNA. It promotes CRC proliferation, invasion, glycolysis and in vivo tumor growth while suppressing apoptosis, mainly through sponging miR-296-3p to up-regulate PGK1. The study suggests circ_0087862 may be an exploitable therapeutic target for CRC. |
| Confidence score: |
0.8571 |