circRNA basic information
circBase ID: hsa_circ_0006168
Name: hsa_circ_CNOT6L
Synonym: hsa_circRNA_103670
Host Gene: CNOT6L
Genomic location(hg19): chr4:78694234-78697546:-
Genomic location(hg38): chr4:77773080-77776392:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0018177
MONDO name: glioblastoma
Disease details: glioblastoma
Disease DO ID:
 3068
Disease MeSH ID:
 D005909
Disease NCIt ID:
 C3058
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

primary GBM tissues; normal brain tissues; xenograft tumor tissues
Cell lines:

A172; LN229; HEB; 293 T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: IGF1R, Ras, Erk1/2, p-Erk1/2, E-cadherin, Vimentin, Snail
Associated microRNA: miR-628-5p
Biological function: hsa_circ_0006168 promotes GBM cell proliferation, colony formation, migration, invasion, EMT and xenograft tumor growth, while inhibiting apoptosis; knockdown suppresses these malignant phenotypes.
Molecular mechanism: hsa_circ_0006168 acts as a ceRNA sponge for miR-628-5p, thereby regulating IGF1R and activating the IGF1R/Ras/Erk signaling pathway.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); apoptosis (inhibits); ERK (promotes); ceRNA regulation (promotes); other pathway/process (promotes)
Detected method:
Q
M
Validation methods:

RNase R Treatment; RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; Luciferase Reporter Assay; RNA Pull-Down; In Vivo Animal Model; Bioinformatics Analysis
Clinical significance:

hsa_circ_0006168 expression was significantly associated with WHO grade, T stage, N stage and M stage in GBM cases, suggesting potential therapeutic value.
Description:

hsa_circ_0006168 is up-regulated in human GBM tissues and cell lines. It promotes GBM proliferation, migration, invasion, colony formation, EMT and xenograft tumor growth, while inhibiting apoptosis. Mechanistically, hsa_circ_0006168 sponges miR-628-5p to regulate IGF1R and activate the Ras/Erk signaling pathway.
Confidence score:

0.7595
Other information
Title:

Blocking hsa_circ_0006168 suppresses cell proliferation and motility of human glioblastoma cells by regulating hsa_circ_0006168/miR-628-5p/IGF1R ceRNA axis.

Journal: Cell cycle (Georgetown, Tex.)
Published: 2021
PubMed ID: 34024251
Study type:

combined biological and clinical study
Data availability: GSE146463
Code availability: -