| Expression pattern: |
UP |
| Associated gene: |
CCND1, CyclinD1 |
| Associated microRNA: |
miR-432, miR-335, miR-432-5p |
| Biological function: |
circATP2C1 promotes BC oncogenesis, tumor growth, proliferation, invasion and metastasis, inhibits apoptosis, and is associated with poor overall survival; circATP2C1 knockdown suppresses BC cell viability, colony proliferation, invasion and in vivo tumor formation while increasing apoptosis. |
| Molecular mechanism: |
circATP2C1 acts as a ceRNA that sponges miR-432 and miR-335, relieving their inhibition of CCND1 and upregulating CCND1 expression to promote breast cancer progression. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); invasion (promotes); metastasis (promotes); apoptosis (inhibits); cell cycle (promotes); EMT (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Microarray; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Transwell Assay; Western Blot; In Vivo Animal Model; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circATP2C1 expression is associated with shorter overall survival and poor prognosis in BC patients; circATP2C1 may serve as a biomarker for early diagnosis and a therapeutic target for BC treatment. |
| Description: |
circATP2C1 is up-regulated in breast cancer tissues and cell lines and its high expression is linked to poorer overall survival. Functionally, circATP2C1 promotes BC cell viability, proliferation, invasion, tumor growth and metastasis while suppressing apoptosis. Mechanistically, it functions as a ceRNA by sponging miR-432/miR-335 to increase CCND1 expression. |
| Confidence score: |
0.8571 |