| Expression pattern: |
UP |
| Associated gene: |
ELK1 |
| Associated microRNA: |
miR-340-3p |
| Biological function: |
promotes proliferation, migration, and invasion of LSCC cells; accelerates tumor growth in vivo |
| Molecular mechanism: |
ceRNA mechanism: circPPFIA1 sponges miR-340-3p, relieving miR-340-3p-mediated inhibition of ELK1 |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
Bioinformatics Analysis; RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; MTT; Colony Formation Assay; Transwell Assay; Luciferase Reporter Assay; RNA Pull-Down; FISH / smFISH; Transfection; In Vivo Animal Model; IHC (Immunohistochemistry); RNA-seq |
| Clinical significance: |
may serve as a novel prognostic or therapeutic target for LSCC |
| Description: |
circPPFIA1 is up-regulated in LSCC tissues and cell lines and promotes malignant phenotypes (proliferation, migration, invasion) and tumor growth. It functions as a ceRNA by sponging miR-340-3p, thereby relieving repression of ELK1 and driving LSCC progression via the miR-340-3p/ELK1 axis. |
| Confidence score: |
0.8195 |