circRNA basic information
circBase ID: hsa_circ_0033351
Name: hsa_circ_DYNC1H1
Synonym: circ_DYNC1H1
Host Gene: DYNC1H1
Genomic location(hg19): chr14:102499401-102507010:+
Genomic location(hg38): chr14:102033064-102040673:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma
Disease DO ID:
 684, 686
Disease MeSH ID:
 D006528
Disease NCIt ID:
 C3099
Disease ICD11 ID:
 1294035808
Disease OMIM ID:
 114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

HCC tissues; normal adjacent tissues; tumor tissues
Cell lines:

Hep3B; Huh7; THLE-2
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: USP14
Associated microRNA: miR-520a-3p
Biological function: promotes proliferation, migration and invasion and inhibits apoptosis in HCC cells; mediates the antitumor effect of lidocaine
Molecular mechanism: ceRNA sponge: circ_DYNC1H1 sponges miR-520a-3p to regulate USP14 expression
Biological pathway or process:

proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; Transfection; Colony Formation Assay; MTT; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Clinical Sample Validation; Bioinformatics Analysis
Clinical significance:

-
Description:

circ_DYNC1H1 is upregulated in HCC and mainly localized in the cytoplasm. It promotes HCC malignant phenotypes by sponging miR-520a-3p and thereby increasing USP14 expression, and lidocaine suppresses HCC progression partly by downregulating circ_DYNC1H1 to modulate the miR-520a-3p/USP14 axis.
Confidence score:

0.8174
Other information
Title:

Lidocaine has antitumor effect on hepatocellular carcinoma via the circ_DYNC1H1/miR-520a-3p/USP14 axis.

Journal: Open life sciences
Published: 2021
PubMed ID: 34435133
Study type:

combined biological and clinical study
Data availability: The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.
Code availability: -