circRNA basic information
circBase ID: -
Name: hsa_circ_HIPK2
Synonym: circular RNA HIPK2
Host Gene: HIPK2
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer
Disease DO ID:
 3908
Disease MeSH ID:
 D002289
Disease NCIt ID:
 C2926
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissue; matched normal tissues
Cell lines:

A549; H460; A549/DDP; H460/DDP; HUVECs
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: VEGFA, Ago2
Associated microRNA: miR-1249-3p
Biological function: Promotes DDP resistance, proliferation, migration, invasion, anti-apoptosis, angiogenesis, and in vivo tumor growth in NSCLC.
Molecular mechanism: circHIPK2 acts as a miRNA sponge for miR-1249-3p and upregulates VEGFA, thereby promoting angiogenesis and chemoresistance in NSCLC.
Biological pathway or process:

chemoresistance (promotes); angiogenesis (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); ceRNA regulation (other)
Detected method:
Q
Validation methods:

RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Tube Formation Assay; Luciferase Reporter Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Survival Analysis; Bioinformatics Analysis
Clinical significance:

High circHIPK2 expression predicts shorter overall survival and is associated with lymph node metastasis and advanced TNM stage; lower circHIPK2 expression is an independent factor predicting prolonged OS.
Description:

circHIPK2 is up-regulated in NSCLC, especially DDP-resistant NSCLC, and high expression is associated with poor overall survival, lymph node metastasis, and advanced TNM stage. Functionally, circHIPK2 promotes DDP chemoresistance, malignant behaviors, angiogenesis, and xenograft growth. Mechanistically, cytoplasmic circHIPK2 sponges miR-1249-3p to upregulate VEGFA.
Confidence score:

0.8712
Other information
Title:

The malignant property of circHIPK2 for angiogenesis and chemoresistance in non-small cell lung cancer.

Journal: Experimental cell research
Published: 2022
PubMed ID: 35863454
Study type:

combined biological and clinical study
Data availability: The data are available from the corresponding author upon request.
Code availability: -