| Expression pattern: |
UP |
| Associated gene: |
FOXO3 |
| Associated microRNA: |
miR-1281 |
| Biological function: |
Promotes NSCLC tumorigenesis by enhancing proliferation, migration and invasion and by suppressing pyroptosis; knockdown induces pyroptosis and inhibits tumor growth in vivo. |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-1281, thereby relieving miR-1281-mediated repression of FOXO3 and modulating pyroptosis-related proteins/cytokines (cleaved caspase 1, GSDME-N, IL-1beta, IL-18). |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); pyroptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Transfection; CCK8; Annexin V/PI Flow Cytometry; RNA Pull-Down; Luciferase Reporter Assay; Western Blot; ELISA; Transwell Assay; Wound Healing Assay; In Vivo Animal Model |
| Clinical significance: |
- |
| Description: |
hsa_circ_0000729 is up-regulated in NSCLC cell lines and acts as an oncogenic circRNA. Its knockdown suppresses proliferation, migration and invasion and induces pyroptosis, partly via the miR-1281/FOXO3 axis, and also inhibits tumor growth in a xenograft model. |
| Confidence score: |
0.6001 |