circRNA basic information
circBase ID: -
Name: hsa_circ_SATB2
Synonym: CircSATB2
Host Gene: SATB2
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0009807
MONDO name: osteosarcoma
Disease details: osteosarcoma
Disease DO ID:
 3347
Disease MeSH ID:
 D012516
Disease NCIt ID:
 C9145
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

OS tissues; adjacent normal tissues; xenograft tumors; lung metastatic nodules
Cell lines:

MG63; U2OS; 143B; SJSA; HOS; hFOB 1.19; hFOB
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: FUS, ZNFX1 mRNA, AGO2
Associated microRNA: miR-661
Biological function: Promotes osteosarcoma cell proliferation, migration and invasion in vitro, and aggravates osteosarcoma growth and lung metastasis in vivo.
Molecular mechanism: circSATB2 promotes osteosarcoma progression by sponging miR-661 and interacting with the RNA-binding protein FUS to regulate and stabilize ZNFX1 mRNA.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (promotes); mRNA stability (promotes); other pathway/process (promotes)
Detected method:
Q
H
S
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; RNA-seq; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Wound Healing Assay; Transwell Assay; IHC (Immunohistochemistry); H&E Staining; Western Blot; In Vivo Animal Model; Cohort Study; Survival Analysis; Bioinformatics Analysis
Clinical significance:

High expression of circSATB2 is related to poor overall survival and correlated with Enneking stage and distant metastasis; circSATB2 may serve as a prognostic marker and therapeutic target for osteosarcoma.
Description:

circSATB2 is up-regulated in osteosarcoma tissues and cell lines, and its high expression is associated with poor overall survival, advanced Enneking stage, and distant metastasis. Functionally, circSATB2 promotes osteosarcoma proliferation, migration, invasion, tumor growth, and lung metastasis. Mechanistically, it acts through a miR-661/ZNFX1 ceRNA route and also interacts with FUS to regulate/stabilize ZNFX1 mRNA.
Confidence score:

0.8981
Other information
Title:

Circular RNA CircSATB2 facilitates osteosarcoma progression through regulating the miR-661/FUS-mediated mRNA of ZNFX1.

Journal: Cellular signalling
Published: 2024
PubMed ID: 37984605
Study type:

combined biological and clinical study
Data availability: online repositories; Supplementary data: https://doi.org/10.1016/j.cellsig.2023.110977
Code availability: -