| Expression pattern: |
DN |
| Associated gene: |
hnRNPK, p53, SENP2, SUMO1, Serpine1, p21 |
| Associated microRNA: |
- |
| Biological function: |
Suppresses colorectal cancer cell proliferation, migration, invasion and metastasis; promotes apoptosis; restrains tumor growth and liver metastasis in vivo. |
| Molecular mechanism: |
Binds hnRNPK (KH3 domain) in the nucleus, enhances hnRNPK SUMOylation by blocking SENP2-mediated deSUMOylation, strengthens hnRNPK-p53 interaction and modulates sequence-specific DNA-binding of the hnRNPK-p53 transcriptional complex, thereby downregulating Serpine1 and upregulating p21. |
| Biological pathway or process: |
proliferation (inhibits); migration (inhibits); invasion (inhibits); metastasis (inhibits); apoptosis (promotes); cell cycle (inhibits); other pathway/process (other) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Sanger Sequencing; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RNA Pull-Down; RIP (RNA Immunoprecipitation); Co-IP; ChIP / ChIP-seq; RNA-seq; Transfection; CCK8; Colony Formation Assay; EdU Staining; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; IHC (Immunohistochemistry); H&E Staining; Survival Analysis; Bioinformatics Analysis; Clinical Sample Validation; Cohort Study |
| Clinical significance: |
Low circ-GALNT16 expression is associated with advanced tumor size/stage and lymph node metastasis, and correlates with shorter overall survival; circ-GALNT16 may serve as a diagnostic and prognostic biomarker for CRC. |
| Description: |
Circ-GALNT16 is downregulated in colorectal cancer and acts as a tumor suppressor. It predominantly localizes to the nucleus, binds hnRNPK (KH3 domain), enhances hnRNPK SUMOylation by blocking SENP2-mediated deSUMOylation, strengthens hnRNPK-p53 transcriptional complex activity, and thereby represses Serpine1 and increases p21, inhibiting proliferation and metastasis and promoting apoptosis. Low circ-GALNT16 is associated with worse clinical features and shorter overall survival. |
| Confidence score: |
0.9026 |