| Expression pattern: |
UP |
| Associated gene: |
E2F2, AGO2 |
| Associated microRNA: |
miR-146b-3p |
| Biological function: |
promotes proliferation, migration and invasion; promotes tumorigenesis and metastasis; inhibits apoptosis; regulates cell cycle |
| Molecular mechanism: |
Acts as a ceRNA sponge for miR-146b-3p to relieve repression of E2F2 |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); apoptosis (inhibits); cell cycle (other) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Sanger Sequencing; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Transwell Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Western Blot; In Vivo Animal Model; H&E Staining; Clinical Sample Validation; Bioinformatics Analysis |
| Clinical significance: |
circ_RPPH1 level was related to clinical stage and pathological grade; high circ_RPPH1 associated with higher TNM stage (III + IV) and increased lymphatic metastasis |
| Description: |
circ_RPPH1 is up-regulated in breast cancer and promotes proliferation, migration/invasion, tumor growth and metastasis. It mainly localizes to the cytoplasm and functions as a ceRNA by sponging miR-146b-3p, thereby relieving repression of E2F2 and driving BC progression. |
| Confidence score: |
0.8519 |