circRNA basic information
circBase ID: -
Name: hsa_circ_ELMO1
Synonym: hsa_circRNA_104348
Host Gene: ELMO1
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma
Disease DO ID:
 684, 686
Disease MeSH ID:
 D006528
Disease NCIt ID:
 C3099
Disease ICD11 ID:
 1294035808
Disease OMIM ID:
 114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

HCC tissue; adjacent normal tissues; peritumor tissues; tumor tissue; liver tissue; lung tissue
Cell lines:

Hep3B; SK-HEP-1; MHCC97L; MHCC97H; Huh7; LO2; HEK293; HEK-293T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: RTKN2, Ago2, beta-catenin, PCNA, CyclinD1, c-Myc, Bcl-2, Bax, MMP-7, E-cadherin, N-cadherin, p-GSK-3beta, LEF1
Associated microRNA: miR-187-3p
Biological function: promotes proliferation, migration, and invasion; suppresses apoptosis; promotes tumorigenesis and lung metastasis
Molecular mechanism: Acts as a ceRNA (miRNA sponge) for miR-187-3p to upregulate RTKN2 and activate Wnt/beta-catenin signaling.
Biological pathway or process:

Wnt/beta-catenin (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); metastasis (promotes); EMT (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; Colony Formation Assay; EdU Staining; Annexin V/PI Flow Cytometry; Wound Healing Assay; Transwell Assay; IF (Immunofluorescence); Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); H&E Staining
Clinical significance:

High hsa_circRNA_104348 level is associated with poor prognosis in HCC patients.
Description:

hsa_circRNA_104348 (has_circ_104348) is up-regulated in hepatocellular carcinoma tissues and cell lines and is associated with worse overall survival. Functionally, it promotes HCC cell proliferation, migration, invasion and EMT-like changes while inhibiting apoptosis, and its knockdown suppresses tumor growth and lung metastasis in xenograft models. Mechanistically, it mainly localizes to the cytoplasm and acts as a ceRNA sponging miR-187-3p to increase RTKN2 and activate Wnt/beta-catenin signaling.
Confidence score:

0.8428
Other information
Title:

CircRNA hsa_circRNA_104348 promotes hepatocellular carcinoma progression through modulating miR-187-3p/RTKN2 axis and activating Wnt/beta-catenin pathway.

Journal: Cell death & disease
Published: 2020
PubMed ID: 33311442
Study type:

combined biological and clinical study
Data availability: All data generated or analyzed during this study are included in this published article.
Code availability: -