| Expression pattern: |
UP |
| Associated gene: |
JAG1, Ago2 |
| Associated microRNA: |
miR-485-3p |
| Biological function: |
Facilitates cell growth/proliferation, cell cycle progression, migration, invasion, angiopoiesis, and tumor growth in osteosarcoma. |
| Molecular mechanism: |
Acts as a miRNA sponge (ceRNA) for miR-485-3p to upregulate JAG1 expression. |
| Biological pathway or process: |
proliferation (promotes); cell cycle (promotes); migration (promotes); invasion (promotes); angiogenesis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Bioinformatics Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; MTT; Cell Cycle Assay; Wound Healing Assay; Transwell Assay; Tube Formation Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Clinical Sample Validation |
| Clinical significance: |
circ_0084582 is likely to be used as a favorable diagnostic and therapeutic indicator in OS. |
| Description: |
circ_0084582 is up-regulated in osteosarcoma tissues and cell lines and mainly localizes to the cytoplasm. It promotes osteosarcoma malignant behaviors (proliferation/cell cycle progression, migration, invasion, and angiopoiesis) and tumor growth by sponging miR-485-3p to increase JAG1 expression. |
| Confidence score: |
0.8325 |