| Expression pattern: |
UP |
| Associated gene: |
WNT4 |
| Associated microRNA: |
miR-637 |
| Biological function: |
Suppresses proliferation and migration of ox-LDL-treated endothelial cells; aggravates ox-LDL-induced endothelial cell injury/dysfunction in an in vitro atherosclerosis model. |
| Molecular mechanism: |
Acts as a ceRNA/miRNA sponge for miR-637, thereby regulating the downstream target WNT4 in ox-LDL-treated endothelial cells. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (inhibits); migration (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; Actinomycin D / DRB Stability Assay; divergent primers PCR; RT-qPCR; FISH / smFISH; Luciferase Reporter Assay; RNA Pull-Down; Transfection; CCK8; Transwell Assay; Wound Healing Assay; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
circUSP36 to be a promising biomarker for the diagnosis and therapy of atherosclerosis. |
| Description: |
In ox-LDL-induced endothelial dysfunction (an in vitro atherosclerosis model), circUSP36 is up-regulated and functions as a miRNA sponge for miR-637. Through the circUSP36/miR-637/WNT4 axis, circUSP36 modulates endothelial cell proliferation and migration and is proposed as a potential diagnostic/therapeutic biomarker for atherosclerosis. |
| Confidence score: |
0.7812 |