| Expression pattern: |
UP |
| Associated gene: |
PDCD4, P53 |
| Associated microRNA: |
miR-330-3p |
| Biological function: |
Inhibits breast cancer cell viability, proliferation, and tumor growth. |
| Molecular mechanism: |
Acts as a ceRNA/miRNA sponge for miR-330-3p, relieving repression of PDCD4 and affecting P53 protein level via the miR-330-3p/PDCD4 axis. |
| Biological pathway or process: |
proliferation (inhibits); ceRNA regulation (promotes); p53 signaling (inhibits) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Luciferase Reporter Assay; Bioinformatics Analysis; Transfection; MTT; Colony Formation Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Clinical Sample Validation |
| Clinical significance: |
hsa_circ_0053063 high expression was only positively related to the age of these patients; may serve as a diagnostic biomarker for aged BC patients. |
| Description: |
hsa_circ_0053063 is up-regulated in breast cancer tissues/cell lines but functions as an anti-oncogenic circRNA. It sponges miR-330-3p to de-repress PDCD4, thereby inhibiting breast cancer cell proliferation and suppressing xenograft tumor growth; it also modulates P53 protein levels via the miR-330-3p/PDCD4 axis. |
| Confidence score: |
0.8277 |