| Expression pattern: |
DN |
| Associated gene: |
H2AX, gammaH2AX, p53, Bclaf1, Bcl2 |
| Associated microRNA: |
- |
| Biological function: |
Inhibits proliferation and survival and promotes apoptosis in p53 mutant breast cancer cells; represses tumor growth and increases mouse survival. |
| Molecular mechanism: |
Forms protein complexes: in p53 wild-type cells circ-Ccnb1 binds H2AX and p53; in p53 mutant cells circ-Ccnb1 forms a complex with H2AX and Bclaf1 (not mutant p53), inducing cell death. |
| Biological pathway or process: |
proliferation (inhibits); apoptosis (promotes); other pathway/process (other) |
| Detected method: |
Q
B
M
|
| Validation methods: |
Microarray; RNase R Treatment; RT-qPCR; Northern Blot; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; RNA Pull-Down; RIP (RNA Immunoprecipitation); CLIP; Western Blot; Transfection; MTT; Annexin V/PI Flow Cytometry; Colony Formation Assay; In Vivo Animal Model; Survival Analysis; ISH (In Situ Hybridization) |
| Clinical significance: |
circ-Ccnb1 levels were decreased in breast carcinoma tissues and were significantly lower in p53 mutation tumors compared to tumors of wild-type p53. |
| Description: |
circ-Ccnb1 is down-regulated in breast carcinoma tissues. Functionally, circ-Ccnb1 suppresses growth and promotes apoptosis of p53-mutant breast cancer cells by forming a circ-Ccnb1-H2AX-Bclaf1 complex, thereby inhibiting xenograft tumor growth and prolonging mouse survival. |
| Confidence score: |
0.8687 |