circRNA basic information
circBase ID: hsa_circ_0089153
Name: -
Synonym: circ_0089153
Host Gene: -
Genomic location(hg19): chr9:134011326-134022971:+
Genomic location(hg38): chr9:131135939-131147584:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007254
MONDO name: breast cancer
Disease details: breast cancer
Disease DO ID:
 1612
Disease MeSH ID:
 -
Disease NCIt ID:
 C9335
Disease ICD11 ID:
 1047754165
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissues; adjacent normal tissues; xenograft tumor tissues; lung tissues
Cell lines:

T47D; MCF-7; BT549; BT-549; MDA-MB-231; MCF10A; MCF-10A
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: E2F6, Ago2
Associated microRNA: miR-2467-3p
Biological function: circ_0089153 acts as an oncogene in breast cancer and promotes BCa cell proliferation, migration, invasion, EMT, in vivo tumor growth and lung metastasis.
Molecular mechanism: circ_0089153 functions as a cytoplasmic miR-2467-3p sponge, relieving miR-2467-3p-mediated suppression of E2F6 and thereby promoting breast cancer malignant progression.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); metastasis (promotes); ceRNA regulation (promotes)
Detected method:
Q
M
Validation methods:

RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; CCK8; EdU Staining; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Western Blot; Luciferase Reporter Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); IHC (Immunohistochemistry); In Vivo Animal Model; H&E Staining; Cohort Study; Survival Analysis; Bioinformatics Analysis
Clinical significance:

High circ_0089153 expression indicates unfavorable survival in BCa patients and circ_0089153 may be a potential target for BCa targeted treatment.
Description:

circ_0089153 is up-regulated in breast cancer tissues and cell lines, and high expression is associated with poorer long-term survival. Functionally, circ_0089153 promotes proliferation, migration, invasion, EMT, tumor growth and lung metastasis. Mechanistically, it mainly acts as a cytoplasmic sponge for miR-2467-3p, increasing E2F6 expression and driving malignant breast cancer progression.
Confidence score:

0.877
Other information
Title:

circ_0089153 exacerbates breast cancer cells proliferation and metastasis via sponging miR-2467-3p/E2F6.

Journal: Environmental toxicology
Published: 2022
PubMed ID: 35225430
Study type:

combined biological and clinical study
Data availability: GSE165884
Code availability: -