| Expression pattern: |
UP |
| Associated gene: |
BTRC, AGO2, NF-kappaB |
| Associated microRNA: |
miR-583 |
| Biological function: |
Promotes IDD progression by enhancing ECM catabolism, suppressing ECM anabolism, and increasing proinflammatory cytokine production in NP cells. |
| Molecular mechanism: |
Acts as a ceRNA/miRNA sponge for miR-583 to upregulate BTRC, thereby activating the BTRC/NF-kappaB signaling pathway and driving inflammatory and ECM-degradative changes. |
| Biological pathway or process: |
NF-kappaB (promotes); inflammation (promotes); ceRNA regulation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
H
M
|
| Validation methods: |
Microarray; Bioinformatics Analysis; RNase R Treatment; divergent primers PCR; Sanger Sequencing; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; Western Blot; In Vivo Animal Model; H&E Staining; IF (Immunofluorescence) |
| Clinical significance: |
circ-FAM169A is upregulated in degenerative NP tissues and is positively correlated with IDD grade. |
| Description: |
circ-FAM169A is upregulated in degenerative NP tissues and correlates positively with IDD grade. It promotes disc degeneration by sponging miR-583 to relieve repression of BTRC, leading to activation of NF-kappaB-associated inflammatory and ECM-catabolic programs. |
| Confidence score: |
0.8118 |