| Expression pattern: |
UN |
| Associated gene: |
PES1, NOP14, BOP1, BRIX1, NOLC1, AGO2 |
| Associated microRNA: |
miR-4659a/b, miR-3184-3p, miR-5571-5p |
| Biological function: |
confers atheroprotection by inducing apoptosis and inhibiting proliferation in vascular smooth muscle cells and macrophages |
| Molecular mechanism: |
circANRIL binds PES1, impairs exonuclease-mediated pre-rRNA processing, inhibits ribosome biogenesis, induces nucleolar stress and activates p53 |
| Biological pathway or process: |
p53 signaling (promotes); apoptosis (promotes); proliferation (inhibits); mitochondrial function (other); oxidative phosphorylation (other); mRNA stability (other); ribosome biogenesis (inhibits); other pathway/process (other) |
| Detected method: |
Q
M
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RT-qPCR; Microarray; RNase R Treatment; Actinomycin D / DRB Stability Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; Western Blot; Northern Blot; IF (Immunofluorescence); ISH (In Situ Hybridization); IHC (Immunohistochemistry); Clinical Sample Validation; Cohort Study; Bioinformatics Analysis; CCK8 |
| Clinical significance: |
High circANRIL expression is associated with less coronary artery disease (CAD) and CAD-protective 9p21 genotype. |
| Description: |
circANRIL (from the ANRIL/CDKN2B-AS1 locus at 9p21) is associated with CAD-protective genotype and lower CAD burden. Mechanistically, it binds PES1 in the PeBoW complex, inhibits pre-rRNA processing and ribosome biogenesis, triggers nucleolar stress and p53 activation, thereby promoting apoptosis and suppressing proliferation in atherogenesis-relevant human vascular cells. |
| Confidence score: |
0.8913 |