| Expression pattern: |
UP |
| Associated gene: |
STIM1 |
| Associated microRNA: |
miR-326 |
| Biological function: |
circHIPK3 promotes ASMC proliferation and migration and inhibits apoptosis; circHIPK3 knockdown represses proliferation and migration and up-regulates apoptosis in PDGF-induced ASMCs. |
| Molecular mechanism: |
circHIPK3 functions as a cytoplasmic miRNA sponge for miR-326, thereby regulating STIM1 through a ceRNA-like circHIPK3/miR-326/STIM1 axis. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); apoptosis (inhibits); ceRNA regulation (other); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Transwell Assay; Annexin V/PI Flow Cytometry; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
providing a novel insight for the therapeutic target |
| Description: |
This study reports that circHIPK3 is up-regulated in PDGF-induced human ASMCs, a cellular model related to asthma airway remodeling. circHIPK3 promotes ASMC proliferation and migration while suppressing apoptosis, acting through a cytoplasmic ceRNA mechanism involving miR-326 and STIM1. The circHIPK3/miR-326/STIM1 axis is proposed as a potential therapeutic target for asthma-related airway remodeling. |
| Confidence score: |
0.7812 |