| Expression pattern: |
UP |
| Associated gene: |
- |
| Associated microRNA: |
miR-145-5p |
| Biological function: |
Promotes 5-Fu resistance in gastric cancer cells; silencing hsa_circ_0004650 decreases cell survival/proliferation activity and promotes apoptosis under 5-Fu treatment. |
| Molecular mechanism: |
hsa_circ_0004650 functions as a ceRNA/miRNA sponge that targets and negatively regulates miR-145-5p, thereby inhibiting apoptosis and facilitating 5-Fu resistance in gastric cancer cells. |
| Biological pathway or process: |
drug resistance (promotes); chemoresistance (promotes); proliferation (promotes); apoptosis (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
S
|
| Validation methods: |
RT-qPCR; RNA-seq; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; CCK8; Annexin V/PI Flow Cytometry |
| Clinical significance: |
Potential therapeutic target for overcoming 5-Fu resistance in gastric cancer. |
| Description: |
hsa_circ_0004650 is up-regulated in 5-Fu-resistant gastric cancer cell lines. It promotes 5-Fu resistance by sponging and negatively regulating miR-145-5p, thereby enhancing cell survival/proliferation and inhibiting apoptosis under 5-Fu treatment. The study proposes hsa_circ_0004650 as a potential therapeutic target for overcoming 5-Fu resistance in gastric cancer. |
| Confidence score: |
0.5804 |