circRNA basic information
circBase ID: hsa_circ_0015278
Name: hsa_circ_KLHL20
Synonym: -
Host Gene: KLHL20
Genomic location(hg19): chr1:173726114-173744981:+
Genomic location(hg38): chr1:173756975-173775842:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer
Disease DO ID:
3908
Disease MeSH ID:
D002289
Disease NCIt ID:
C2926
Disease ICD11 ID:
-
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

NSCLC tissues; adjacent non-tumor tissues; subcutaneous tumors

Cell lines:

CALU3; A549; H1229; H1975; HBEs

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
DN
Associated gene: SOCS6
Associated microRNA: miR-1278
Biological function: inhibits proliferation, colony formation, invasion, and EMT; induces apoptosis; suppresses tumor growth in vivo
Molecular mechanism: acts as a miR-1278 sponge and promotes SOCS6 expression
Biological pathway or process:

proliferation (inhibits); invasion (inhibits); EMT (inhibits); apoptosis (promotes); ceRNA regulation (promotes)

Detected method:
Q
Validation methods:

Microarray; Bioinformatics Analysis; RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; CCK8; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Clinical Sample Validation; Cohort Study; Survival Analysis

Clinical significance:

reduced expression indicated poor prognosis

Description:

circ_0015278 is down-regulated in NSCLC and acts as a tumor suppressor. It sponges miR-1278 to relieve repression of SOCS6, thereby inhibiting proliferation, invasion and EMT and promoting apoptosis, and it suppresses xenograft tumor growth. Low circ_0015278 expression is associated with worse patient survival.

Confidence score:

0.8824

Other information
Title:

Circular RNA _0015278 inhibits the progression of non-small cell lung cancer through regulating the microRNA 1278/SOCS6 gene axis.

Journal: Annals of translational medicine
Published: 2021
PubMed ID: 34532392
Study type:

combined biological and clinical study

Data availability: GSE112214; GSE158659; GSE101586
Code availability: -