| Expression pattern: |
UP |
| Associated gene: |
Diaphanous Homolog 1, DIAPH1 |
| Associated microRNA: |
miR-665 |
| Biological function: |
Circ_0044556 functions as an oncogenic circRNA in colorectal cancer, promoting cell proliferation, cell cycle progression, migration, invasion, EMT, and tumor growth. |
| Molecular mechanism: |
Circ_0044556 acts as a miR-665 sponge to upregulate DIAPH1 expression, thereby promoting CRC progression. |
| Biological pathway or process: |
proliferation (promotes); cell cycle (promotes); migration (promotes); invasion (promotes); EMT (promotes); metastasis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Actinomycin D / DRB Stability Assay; RNase R Treatment; RT-qPCR; Clinical Sample Validation; Transfection; CCK8; EdU Staining; Cell Cycle Assay; Western Blot; Transwell Assay; Luciferase Reporter Assay; In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
circ_0044556 might be an efficient biomarker to improve the management of diagnosis and treatment in CRC. |
| Description: |
circ_0044556 is up-regulated in colorectal cancer tissues and cell lines and acts as an oncogenic circRNA. It promotes CRC proliferation, cell cycle progression, migration, invasion, EMT, and tumor growth by sponging miR-665 and increasing DIAPH1 expression. The study also suggests circ_0044556 may serve as a biomarker for CRC diagnosis and treatment management. |
| Confidence score: |
0.7595 |