| Expression pattern: |
UP |
| Associated gene: |
PYGM, BMF, MBNL3, DENND6A, REEP5, KLF6, ITM2C |
| Associated microRNA: |
- |
| Biological function: |
promotes proliferation, migration and invasion of cervical cancer cells |
| Molecular mechanism: |
May regulate cervical cancer through downstream gene expression changes after circYPEL2 knockdown (RNA-seq identified regulated genes; enrichment in EMT and KRAS signaling and metabolic/hypoxia-related pathways). |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); EMT (other); KRAS signaling (other); oxidative phosphorylation (other); HIF-1 signaling (other) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; Colony Formation Assay; Transwell Assay |
| Clinical significance: |
potential biomarker for clinical research of cervical cancer |
| Description: |
circYPEL2 (hsa_circ_0005600), derived from YPEL2 exon 2, is up-regulated in cervical cancer and mainly localized in the cytoplasm. Functional assays show circYPEL2 promotes cervical cancer cell proliferation, migration, and invasion; knockdown alters downstream gene expression with enrichment in EMT and KRAS signaling-related programs. |
| Confidence score: |
0.685 |