circRNA basic information
circBase ID: hsa_circ_0082835
Name: hsa_circ_EZH2
Synonym: circRNA_0082835
Host Gene: EZH2
Genomic location(hg19): chr7:148511050-148511229:-
Genomic location(hg38): chr7:148813958-148814137:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005105
MONDO name: melanoma
Disease details: primary melanoma
Disease DO ID:
 1909
Disease MeSH ID:
 D008545
Disease NCIt ID:
 C3224
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

melanoma tissues
Cell lines:

WM451; A375; SK-MEL-24; WM35; HM
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: EZH2, Wnt5a, beta-catenin, CyclinD1, CyclinE1, CRKL, E-cad, N-cad
Associated microRNA: miR-429
Biological function: promotes proliferation, invasion and migration; regulates cell cycle; promotes EMT; promotes lymphatic metastasis
Molecular mechanism: ceRNA (miRNA sponge): circRNA_0082835 binds/sponges miR-429, reducing its anti-tumor effects and affecting Wnt/beta-catenin signaling and EMT-related proteins.
Biological pathway or process:

Wnt/beta-catenin (promotes); EMT (promotes); proliferation (promotes); invasion (promotes); migration (promotes); cell cycle (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Cell Cycle Assay; Transwell Assay; Wound Healing Assay; Western Blot; Luciferase Reporter Assay; Bioinformatics Analysis
Clinical significance:

circRNA_0082835 expression increased with higher lymphatic metastasis level (number of metastatic lymph nodes).
Description:

circRNA_0082835 is up-regulated in melanoma and increases with lymphatic metastasis. It promotes melanoma cell proliferation, invasion, migration, cell-cycle progression, Wnt/beta-catenin activity and EMT by sponging miR-429, thereby counteracting miR-429’s anti-tumor effects.
Confidence score:

0.6536
Other information
Title:

Circular RNA circRNA_0082835 promotes progression and lymphatic metastasis of primary melanoma by sponging microRNA miRNA-429.

Journal: Bioengineered
Published: 2021
PubMed ID: 34288815
Study type:

combined biological and clinical study
Data availability: The data that support the findings of this study are available from the corresponding author upon reasonable request.
Code availability: -