circRNA basic information
circBase ID: hsa_circ_0003489
Name: hsa_circ_CDK8
Synonym: circCDK8
Host Gene: CDK8
Genomic location(hg19): chr13:26974589-26975761:+
Genomic location(hg38): chr13:26400452-26401624:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005076
MONDO name: periodontitis
Disease details: periodontitis
Disease DO ID:
 824, 9893
Disease MeSH ID:
 D010518
Disease NCIt ID:
 C34918
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

PDL tissues; periodontitis tissues; healthy PDL tissues; inflamed PDL tissues
Cell lines:

PDLSCs; Human PDLSCs
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: mTOR signaling, p-mTOR, ATG5, LC3, Bcl-2, caspase-3
Associated microRNA: -
Biological function: circCDK8 represses osteogenic differentiation of PDLSCs under hypoxia, induces autophagy and apoptosis, and circCDK8 silencing reverses the inhibitory effect of CoCl2 on osteogenic differentiation.
Molecular mechanism: circCDK8 induces ER stress/autophagy and apoptosis through mTOR signaling during hypoxia, thereby inhibiting osteogenic differentiation of PDLSCs.
Biological pathway or process:

autophagy (promotes); apoptosis (promotes); other pathway/process (inhibits)
Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Transfection; CCK8; IF (Immunofluorescence); Annexin V/PI Flow Cytometry; Western Blot; Bioinformatics Analysis
Clinical significance:

CircCDK8 could be a new therapeutic target of periodontitis.
Description:

This study identifies hsa_circ_0003489/circCDK8 as an up-regulated circRNA in periodontitis tissues and in CoCl2-induced hypoxic PDLSCs. Functionally, circCDK8 promotes ER stress/autophagy and apoptosis through mTOR signaling, thereby repressing osteogenic differentiation of PDLSCs under hypoxia. Silencing circCDK8 reverses hypoxia-induced inhibition of osteogenesis, suggesting circCDK8 as a potential therapeutic target for periodontitis.
Confidence score:

0.5732
Other information
Title:

CircCDK8 regulates osteogenic differentiation and apoptosis of PDLSCs by inducing ER stress/autophagy during hypoxia.

Journal: Annals of the New York Academy of Sciences
Published: 2021
PubMed ID: 32978798
Study type:

combined biological and clinical study
Data availability: -
Code availability: GeneMANIA (http://genemania.org/)