circRNA basic information
circBase ID: hsa_circ_0018189
Name: hsa_circ_CUL2
Synonym: circCUL2
Host Gene: CUL2
Genomic location(hg19): chr10:35321362-35338693:-
Genomic location(hg38): chr10:35032434-35049765:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer / NSCLC
Disease DO ID:
 3908
Disease MeSH ID:
 D002289
Disease NCIt ID:
 C2926
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

NSCLC tumor tissues; adjacent normal tissues; xenograft tumor tissues
Cell lines:

BEAS2B; HCC44; A549; 293 T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: xCT / SLC7A11
Associated microRNA: miR-656-3p
Biological function: promotes NSCLC cell proliferation, migration, invasion, glutamine metabolism and tumor growth in vivo; inhibits apoptosis
Molecular mechanism: Acts as a miR-656-3p sponge to up-regulate xCT (SLC7A11) expression, enhancing NSCLC malignancy and glutamine metabolism
Biological pathway or process:

proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); EMT (promotes); glutaminolysis (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; ELISA; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; IHC (Immunohistochemistry); RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; In Vivo Animal Model
Clinical significance:

-
Description:

hsa_circ_0018189 (circCUL2) is up-regulated in NSCLC tissues and cell lines and promotes proliferation, migration/invasion, EMT-like changes, glutamine metabolism, and xenograft tumor growth. Mechanistically, it acts as a ceRNA by sponging miR-656-3p, thereby increasing xCT (SLC7A11) expression.
Confidence score:

0.8327
Other information
Title:

Circular RNA hsa_circ_0018189 drives non-small cell lung cancer growth by sequestering miR-656-3p and enhancing xCT expression.

Journal: Journal of clinical laboratory analysis
Published: 2022
PubMed ID: 36164726
Study type:

combined biological and clinical study
Data availability: Data sharing is not applicable to this article as no datasets were generated or analyzed during the current study.
Code availability: -