| Expression pattern: |
DN |
| Associated gene: |
SOCS3, FAK/PI3K/AKT pathway |
| Associated microRNA: |
miR-30c-5p |
| Biological function: |
circMTO1 suppresses fibroblast-myofibroblast transition, migration, and invasion in arecoline-treated buccal mucosal fibroblasts and inhibits OSF development. |
| Molecular mechanism: |
circMTO1 acts as a miR-30c-5p sponge to increase SOCS3 expression, leading to inactivation of the FAK/PI3K/AKT pathway. |
| Biological pathway or process: |
PI3K/AKT (inhibits); migration (inhibits); invasion (inhibits); fibrosis (inhibits); ceRNA regulation (promotes); other pathway/process (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; FISH / smFISH; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; Wound Healing Assay; Transwell Assay; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
circMTO1 could be considered a therapeutic target for delaying the OSF process. |
| Description: |
circMTO1 is down-regulated in OSF tissues and arecoline-stimulated BMFs. Its overexpression suppresses fibroblast-myofibroblast transition, migration, and invasion by sponging miR-30c-5p to enhance SOCS3 expression and inactivate the FAK/PI3K/AKT pathway, suggesting a potential therapeutic role in OSF. |
| Confidence score: |
0.7944 |