| Expression pattern: |
UP |
| Associated gene: |
MEX3C, Ago2 |
| Associated microRNA: |
miR-136 |
| Biological function: |
promotes cervical cancer cell proliferation, invasion, sphere formation (CSC-like properties), tumor growth in vivo, and paclitaxel resistance |
| Molecular mechanism: |
ceRNA mechanism: circ_0004488 sponges miR-136 to increase MEX3C expression, thereby enhancing CSC-like features and paclitaxel resistance |
| Biological pathway or process: |
proliferation (promotes); invasion (promotes); stemness (promotes); chemoresistance (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
divergent primers PCR; RNase R Treatment; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Transwell Assay; Western Blot; In Vivo Animal Model; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circ_0004488 expression is associated with poor prognosis and worse overall survival in cervical cancer patients. |
| Description: |
circ_0004488 is up-regulated in cervical cancer (especially in CSC-enriched spheres and paclitaxel-resistant cells/tissues) and promotes proliferation, invasion, sphere formation, and paclitaxel resistance. Mechanistically, it mainly localizes to the cytoplasm and functions as a ceRNA by sponging miR-136 to upregulate MEX3C, enhancing CSC-like traits and chemoresistance. |
| Confidence score: |
0.8562 |