| Expression pattern: |
UP |
| Associated gene: |
HuR |
| Associated microRNA: |
- |
| Biological function: |
Promotes HCC cell proliferation, migration, invasion and EMT; supports tumor growth and exosome-mediated macrophage M2 polarization; its knockdown induces apoptosis and suppresses these malignant phenotypes. |
| Molecular mechanism: |
Binds the RNA-binding protein HuR and reduces HuR protein stability, suppressing EMT (including ZEB-1/vimentin/E-cadherin changes); additionally, circRNA is transferred via exosomes to macrophages to inhibit M2 polarization, thereby reducing pro-migratory/invasive effects on HCC cells. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); EMT (promotes); macrophage polarization (promotes); immune regulation (other pathway/process) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; Bioinformatics Analysis; RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Transwell Assay; Annexin V/PI Flow Cytometry; Western Blot; RNA Pull-Down; FISH / smFISH; In Vivo Animal Model; IHC (Immunohistochemistry) |
| Clinical significance: |
Potential diagnostic and therapeutic relevance for HCC. |
| Description: |
This study reports that hsa_circ_0074854 (also referred to as hsa_circ_00074854) is up-regulated in HCC and promotes malignant phenotypes including proliferation, migration, invasion and EMT. Its knockdown reduces HuR protein stability via direct interaction and also limits exosome-mediated macrophage M2 polarization, together suppressing HCC progression in vitro and in xenograft models. |
| Confidence score: |
0.7486 |