circRNA basic information
circBase ID: hsa_circ_0120472
Name: hsa_circ_CCDC85A
Synonym: circCCDC85A
Host Gene: CCDC85A
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007254
MONDO name: breast cancer
Disease details: breast cancer
Disease DO ID:
 1612
Disease MeSH ID:
 -
Disease NCIt ID:
 C9335
Disease ICD11 ID:
 1047754165
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

breast cancer tissues; normal breast tissues
Cell lines:

MDA-MB-231; BT-549; MCF-7
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: MOB1A, AGO2
Associated microRNA: miR-550a-5p
Biological function: inhibits proliferation, migration, and invasion of breast cancer cells
Molecular mechanism: Acts as a molecular sponge (ceRNA) for miR-550a-5p to restore miR-550a-5p-mediated repression and enhance MOB1A expression.
Biological pathway or process:

proliferation (inhibits); migration (inhibits); invasion (inhibits); ceRNA regulation (promotes); Hippo/YAP (other)
Detected method:
Q
H
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Western Blot; In Vivo Animal Model
Clinical significance:

-
Description:

circCCDC85A is down-regulated in breast cancer and suppresses malignant phenotypes (proliferation, migration, invasion). It primarily functions as a ceRNA by sponging miR-550a-5p, thereby relieving miR-550a-5p-mediated repression and increasing MOB1A expression, with links to Hippo signaling components via the miR-550a-5p target MOB1A.
Confidence score:

0.8231
Other information
Title:

Circular RNA circCCDC85A inhibits breast cancer progression via acting as a miR-550a-5p sponge to enhance MOB1A expression.

Journal: Breast cancer research : BCR
Published: 2022
PubMed ID: 34983617
Study type:

combined biological and clinical study
Data availability: The online version contains supplementary material available at 10.1186/s13058-021-01497-6.
Code availability: -