circRNA basic information
circBase ID: hsa_circ_0057021
Name: hsa_circ_TLK1
Synonym: circTLK1
Host Gene: TLK1
Genomic location(hg19): chr2:171847332-171902872:-
Genomic location(hg38): chr2:170990822-171046362:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0008978
MONDO name: chordoma
Disease details: chordoma
Disease DO ID:
 3302
Disease MeSH ID:
 D002817
Disease NCIt ID:
 C2947
Disease ICD11 ID:
 898231522
Disease OMIM ID:
 215400
Species: Human
Species details: Homo sapiens
Tissue specimen:

Chordoma; pulposus tissue
Cell lines:

U-CH1; MUG-Chor1
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: Smad3, AGO2, TLK1
Associated microRNA: miR-16-5p
Biological function: Promotes chordoma cell proliferation, migration/invasion and EMT; enhances tumor growth in vivo.
Molecular mechanism: Acts as a miRNA sponge for miR-16-5p to enhance Smad3 expression; forms a positive feedback loop where Smad3 promotes TLK1/circTLK1 transcription; interaction involves AGO2.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

Bioinformatics Analysis; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; Agarose gel electrophoresis; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Clinical Sample Validation; Transfection; CCK8; EdU Staining; Wound Healing Assay; Transwell Assay; Western Blot; Luciferase Reporter Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); ChIP / ChIP-seq; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Survival Analysis
Clinical significance:

-
Description:

circTLK1 is up-regulated in chordoma tissues and cell lines and promotes malignant phenotypes (proliferation, migration/invasion and EMT) and xenograft tumor growth. Mechanistically, it sponges miR-16-5p in an AGO2-associated complex to increase Smad3, and Smad3 in turn transcriptionally activates TLK1/circTLK1, forming a positive feedback loop.
Confidence score:

0.8968
Other information
Title:

The malignancy of chordomas is enhanced via a circTLK1/miR-16-5p/Smad3 positive feedback axis.

Journal: Cell death discovery
Published: 2023
PubMed ID: 36792585
Study type:

combined biological and clinical study
Data availability: The data that were collected and analyzed during the current study are available from the corresponding author on reasonable request.
Code availability: -