| Expression pattern: |
UP |
| Associated gene: |
EIF4A3, ATG12 |
| Associated microRNA: |
- |
| Biological function: |
Promotes K562/R cell viability, invasion, autophagy, tumor growth and Imatinib resistance, and inhibits apoptosis. |
| Molecular mechanism: |
circ_SIRT1 binds EIF4A3 and regulates EIF4A3-mediated ATG12 expression, thereby promoting autophagy and Imatinib resistance in CML. |
| Biological pathway or process: |
proliferation (promotes); invasion (promotes); apoptosis (inhibits); autophagy (promotes); drug resistance (promotes); mRNA stability (promotes); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; RT-qPCR; FISH / smFISH; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Actinomycin D / DRB Stability Assay; Transfection; CCK8; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
High expression of circ_SIRT1 is correlated with Imatinib resistance in CML and circ_SIRT1 may serve as a novel therapeutic target for Imatinib-resistant CML treatment. |
| Description: |
circ_SIRT1 is a SIRT1-derived human circRNA that is up-regulated in CML, especially in Imatinib-resistant CML samples and K562/R cells. It promotes CML cell viability, invasion, autophagy, xenograft tumor growth and Imatinib resistance while suppressing apoptosis. Mechanistically, circ_SIRT1 binds EIF4A3 and increases ATG12 expression, linking the circ_SIRT1-EIF4A3-ATG12 mechanism to autophagy-associated Imatinib resistance. |
| Confidence score: |
0.8568 |