circRNA basic information
circBase ID: hsa_circ_0015756
Name: hsa_circ_CFH
Synonym: circ-CFH / circ_0015756
Host Gene: CFH
Genomic location(hg19): chr1:196706604-196712758:+
Genomic location(hg38): chr1:196737474-196743628:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma / HCC
Disease DO ID:
 684, 686
Disease MeSH ID:
 D006528
Disease NCIt ID:
 C3099
Disease ICD11 ID:
 1294035808
Disease OMIM ID:
 114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

HCC tissues; paired nontumorous tissues; xenograft tumor tissues
Cell lines:

Huh7; SNU387; THLE-2
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: RNF38, Ago2, GLUT1
Associated microRNA: miR-377-3p
Biological function: Promotes HCC progression by promoting proliferation, migration, invasion, and glycolysis and inhibiting apoptosis; silencing inhibits tumor growth in vivo.
Molecular mechanism: Acts as a miRNA sponge (ceRNA) for miR-377-3p to increase RNF38 expression.
Biological pathway or process:

proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); glycolysis (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; MTT; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; In Vivo Animal Model
Clinical significance:

High circ-CFH expression was associated with high TNM stage and metastasis; suggested as a predictor/diagnostic marker for HCC prognosis.
Description:

circ-CFH is up-regulated in HCC tissues/cells and promotes malignant phenotypes (proliferation, migration, invasion, glycolysis) while inhibiting apoptosis. It functions mainly as a ceRNA sponge for miR-377-3p, thereby increasing RNF38 expression and accelerating HCC progression; circ-CFH silencing suppresses xenograft tumor growth.
Confidence score:

0.7045
Other information
Title:

circ-CFH promotes the development of HCC by regulating cell proliferation, apoptosis, migration, invasion, and glycolysis through the miR-377-3p/RNF38 axis.

Journal: Open life sciences
Published: 2022
PubMed ID: 35415236
Study type:

combined biological and clinical study
Data availability: The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.
Code availability: -