| Expression pattern: |
UP |
| Associated gene: |
TWIST1 |
| Associated microRNA: |
miR-580-3p |
| Biological function: |
promotes osteosarcoma cell proliferation, migration, invasion and tumor growth |
| Molecular mechanism: |
ceRNA sponging miR-580-3p to upregulate TWIST1 |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); tumor growth (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Luciferase Reporter Assay; RNA Pull-Down; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry) |
| Clinical significance: |
High levels of circRAB3IP was correlated with advanced TNM stage, distant metastasis; positively correlated with tumor size |
| Description: |
circRAB3IP is up-regulated in osteosarcoma tissues and cell lines and functions as an oncogenic circRNA. It promotes proliferation, migration, invasion and in vivo tumor growth by sponging miR-580-3p, thereby relieving repression of TWIST1 (circRAB3IP/miR-580-3p/TWIST1 ceRNA axis). |
| Confidence score: |
0.7695 |