| Expression pattern: |
UP |
| Associated gene: |
CEBPB, CD44, EIF3E, CD44 promoter |
| Associated microRNA: |
- |
| Biological function: |
circMYBL1 promotes malignant phenotypes of ACC cells, enhances migration, invasion, sphere formation, colony formation, anoikis resistance, adhesion between ACC cells and pulmonary endothelial cells, tumor cell arrest, and lung metastasis. |
| Molecular mechanism: |
circMYBL1 forms a circRNA-protein complex with CEBPB, inhibits ubiquitin-mediated degradation of CEBPB, promotes CEBPB nuclear translocation, increases CEBPB binding to the CD44 promoter, enhances CD44 transcription, and can be transmitted via sEVs to promote ACC lung metastasis. |
| Biological pathway or process: |
metastasis (promotes); migration (promotes); invasion (promotes); proliferation (promotes); stemness (promotes); ubiquitination (inhibits); other pathway/process (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RNase R Treatment; RT-qPCR; FISH / smFISH; IF (Immunofluorescence); Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Co-IP; ChIP / ChIP-seq; Transfection; Colony Formation Assay; Transwell Assay; Wound Healing Assay; Flow Cytometry（Non-apoptosis/cycle）; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; H&E Staining; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circMYBL1 expression is positively correlated with lung metastasis, tumor-node-metastasis stage, and poor cumulative survival; circMYBL1 may serve as a biomarker and therapeutic target for ACC. |
| Description: |
circMYBL1 is a MYBL1-derived circRNA upregulated in salivary ACC tissues and plasma sEVs, and high expression is associated with lung metastasis and poor survival. It promotes ACC malignant phenotypes and lung metastasis by binding and stabilizing CEBPB, enhancing CEBPB nuclear translocation and CD44 transcription, and by sEV-mediated transfer that increases CD44-dependent adhesion between ACC cells and pulmonary endothelial cells. |
| Confidence score: |
0.8973 |