| Expression pattern: |
UP |
| Associated gene: |
HK2, Hexokinase 2 |
| Associated microRNA: |
miR-125a-5p |
| Biological function: |
promotes glucose metabolism and resistance to oxidative stress-induced cell injury; prevents cell apoptosis in fibroblast-like synoviocytes during rheumatoid arthritis |
| Molecular mechanism: |
circFNDC3B sponges miR-125a-5p to form a ceRNA network, thereby regulating HK2 and glucose metabolism in FLSs-RA |
| Biological pathway or process: |
glycolysis (promotes); apoptosis (inhibits); ceRNA regulation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; RNA Pull-Down; Luciferase Reporter Assay; Transfection; MTT; Annexin V/PI Flow Cytometry; Colony Formation Assay; Western Blot |
| Clinical significance: |
circFNDC3B could serve as a potentially diagnostic biomarker and therapeutic target for RA treatment |
| Description: |
circFNDC3B is upregulated in human RA fibroblast-like synoviocytes. It acts as a ceRNA by sponging miR-125a-5p, thereby regulating HK2, enhancing glucose metabolism, and reducing oxidative stress-induced cell injury/apoptosis in FLSs-RA. The study suggests circFNDC3B as a potential diagnostic biomarker and therapeutic target for rheumatoid arthritis. |
| Confidence score: |
0.7032 |