| Expression pattern: |
UP |
| Associated gene: |
ASCT2, GLS1 |
| Associated microRNA: |
miR-491-5p |
| Biological function: |
Promotes HNSCC cell proliferation, migration, invasion and glutaminolysis, and inhibits apoptosis; high abundance is associated with poor prognosis. |
| Molecular mechanism: |
circMAT2B acts as a ceRNA/miRNA sponge for miR-491-5p, thereby enhancing ASCT2 expression and ASCT2-mediated glutaminolysis in HNSCC cells. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); glutaminolysis (promotes); ceRNA regulation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; TUNEL; Wound Healing Assay; Transwell Assay; Western Blot; Cohort Study; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circMAT2B abundance was positively correlated with poor prognosis and may provide promising diagnostic and prognostic biomarkers for HNSCC. |
| Description: |
circMAT2B is an up-regulated human circRNA in HNSCC tissues and cell lines, and its high expression is associated with poor patient survival. Functionally, it promotes proliferation, migration, invasion and glutaminolysis while suppressing apoptosis, acting through a circMAT2B/miR-491-5p/ASCT2 ceRNA axis. |
| Confidence score: |
0.8595 |