| Expression pattern: |
DN |
| Associated gene: |
PTEN, Ago2 |
| Associated microRNA: |
miR-10a-5p |
| Biological function: |
circPSMC3 suppresses proliferation, promotes apoptosis, and increases gefitinib sensitivity in gefitinib-resistant ESCC cells. |
| Molecular mechanism: |
circPSMC3 acts as a miR-10a-5p sponge and upregulates PTEN, thereby enhancing gefitinib sensitivity in ESCC cells. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (inhibits); apoptosis (promotes); drug resistance (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; MTT; Annexin V/PI Flow Cytometry; Western Blot; Luciferase Reporter Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); Bioinformatics Analysis |
| Clinical significance: |
circPSMC3 may serve as a therapeutic target for overcoming gefitinib resistance in ESCC treatment. |
| Description: |
circPSMC3 is downregulated in ESCC tissues, ESCC cell lines, and gefitinib-resistant ESCC cells. Restoring circPSMC3 suppresses proliferation, promotes apoptosis, and sensitizes resistant ESCC cells to gefitinib through the miR-10a-5p/PTEN axis, suggesting therapeutic potential for overcoming gefitinib resistance. |
| Confidence score: |
0.7244 |