circRNA basic information
circBase ID: hsa_circ_0030586
Name: hsa_circ_ABCC4
Synonym: hsa_circ_003058
Host Gene: ABCC4
Genomic location(hg19): chr13:95813442-95840796:-
Genomic location(hg38): chr13:95161188-95188542:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0008315
MONDO name: prostate cancer
Disease details: prostate cancer
Disease DO ID:
 10283
Disease MeSH ID:
 D011471
Disease NCIt ID:
 C7378
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

-
Cell lines:

RWPE-1; DU145; PC3; 22Rv1; LNCaP; PC-3 M-2B4; PC-3 M-IE8
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: PIK3CB, IKKalpha, AKT, p-AKT, E-cadherin, Twist, ANGPT2, CHUK, CDK2, BRCA1
Associated microRNA: hsa-miR-145-3p
Biological function: promotes proliferation, migration, invasion and epithelial-mesenchymal transition (EMT) in prostate cancer cells
Molecular mechanism: ceRNA/miRNA sponge mechanism involving miR-145-3p, activating PI3K-AKT signaling and EMT
Biological pathway or process:

PI3K/AKT (promotes); EMT (promotes); proliferation (promotes); migration (promotes); invasion (promotes); ceRNA regulation (promotes)
Detected method:
Q
M
Validation methods:

divergent primers PCR; Sanger Sequencing; RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Transwell Assay; Wound Healing Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; H&E Staining; Bioinformatics Analysis; RNA-seq; Microarray
Clinical significance:

It is suggested that hsa_circ_0030586 can be used as a molecular marker for PCa, helping its early diagnosis and treatment.
Description:

hsa_circ_0030586 is up-regulated in prostate cancer cell lines and promotes proliferation, migration, invasion, and EMT. Mechanistically, it likely sponges hsa-miR-145-3p to activate the PI3K/AKT pathway, and miR-145-3p inhibition reverses the effects of hsa_circ_0030586 knockdown.
Confidence score:

0.7859
Other information
Title:

Hsa_circ_0030586 promotes epithelial-mesenchymal transition in prostate cancer via PI3K-AKT signaling.

Journal: Bioengineered
Published: 2021
PubMed ID: 34852706
Study type:

biological research
Data availability: GSE77661
Code availability: -