| Expression pattern: |
UP |
| Associated gene: |
galectin-3 |
| Associated microRNA: |
miR-299-5p |
| Biological function: |
Promotes oxidative stress (H2O2)-induced apoptosis and autophagy and reduces cell viability in nucleus pulposus cells in the context of IDD. |
| Molecular mechanism: |
ceRNA mechanism: circ_RERE sponges miR-299-5p to increase galectin-3 expression, thereby promoting apoptosis and autophagy under oxidative stress. |
| Biological pathway or process: |
apoptosis (promotes); autophagy (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
RT-qPCR; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; CCK8; Annexin V/PI Flow Cytometry; Western Blot; IF (Immunofluorescence); In Vivo Animal Model; Microarray |
| Clinical significance: |
May provide a new target for the clinical treatment of IDD. |
| Description: |
circ_RERE is up-regulated in IDD NP tissue and in H2O2-treated human NP cells. It promotes oxidative stress-induced NP cell apoptosis and autophagy by sponging miR-299-5p and thereby increasing galectin-3 expression. |
| Confidence score: |
0.5997 |