| Expression pattern: |
DN |
| Associated gene: |
EIF4A3, DRAM1, PI3K mRNA, BAX, caspase 3, AKT, p-AKT |
| Associated microRNA: |
miR-324-5p |
| Biological function: |
Promotes BPDE-induced apoptosis during stress injury; inhibits cell viability, proliferation, anchorage-independent growth and migration; suppresses BPDE-induced malignant transformation of human bronchial epithelial cells. |
| Molecular mechanism: |
circ_0067716 forms a double-negative feedback loop with EIF4A3; acts through a ceRNA mechanism involving miR-324-5p/DRAM1 to promote apoptosis; recruits EIF4A3 and reduces PI3K mRNA stability to inhibit PI3K/AKT signaling and proliferation. |
| Biological pathway or process: |
apoptosis (promotes); proliferation (inhibits); migration (inhibits); invasion (inhibits); metastasis (inhibits); PI3K/AKT (inhibits); ceRNA regulation (other); mRNA stability (inhibits); other pathway/process (inhibits) |
| Detected method: |
Q
S
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; MTT; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; IF (Immunofluorescence); Western Blot; ELISA; Bioinformatics Analysis |
| Clinical significance: |
Lower circ_0067716 expression was correlated with smoking, tumor size, TNM stage, and higher BPDE-DNA adduct levels in lung cancer tissues of smokers. |
| Description: |
circ_0067716 is a human RNF13-derived circRNA that is upregulated during acute BPDE-induced stress injury but downregulated during malignant transformation and in lung cancer tissues. It promotes apoptosis via the miR-324-5p/DRAM1/BAX/caspase 3 axis, while its loss facilitates malignant transformation and proliferation through EIF4A3-mediated stabilization of PI3K mRNA and activation of PI3K/AKT signaling. Clinically, lower circ_0067716 expression is associated with smoking-related lung cancer features including smoking status, tumor size, TNM stage, and BPDE-DNA adduct burden. |
| Confidence score: |
0.8343 |