| Expression pattern: |
UP |
| Associated gene: |
COL11A1, AGO2 |
| Associated microRNA: |
miR-20a-3p |
| Biological function: |
promotes PDAC cell proliferation, invasion, migration, tumorigenesis and metastasis; activates EMT; inhibits apoptosis |
| Molecular mechanism: |
ceRNA mechanism: circ-0005105 sponges miR-20a-3p to upregulate COL11A1, leading to EMT activation |
| Biological pathway or process: |
proliferation (promotes); invasion (promotes); migration (promotes); metastasis (promotes); apoptosis (inhibits); EMT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
H
M
|
| Validation methods: |
Microarray; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; ISH (In Situ Hybridization); Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; EdU Staining; Colony Formation Assay; TUNEL; Transwell Assay; Wound Healing Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; H&E Staining; Survival Analysis; Cohort Study |
| Clinical significance: |
Higher circ-0005105 expression correlated positively with the malignant clinical phenotype and poor prognosis of patients with PDAC; potential prognostic marker and therapeutic target |
| Description: |
circ-0005105 is up-regulated in PDAC tissues and cell lines and promotes proliferation, invasion/migration, tumor growth and metastasis. It functions as a ceRNA by sponging miR-20a-3p to upregulate COL11A1, thereby activating EMT, and high circ-0005105 predicts poor survival in PDAC. |
| Confidence score: |
0.8855 |